Fate of xylem-transported 11C- and 13C-labeled CO2 in leaves of poplar.
Identifieur interne : 001D63 ( Main/Exploration ); précédent : 001D62; suivant : 001D64Fate of xylem-transported 11C- and 13C-labeled CO2 in leaves of poplar.
Auteurs : Jasper Bloemen [Belgique] ; Ingvar Bauweraerts ; Filip De Vos ; Christian Vanhove ; Stefaan Vandenberghe ; Pascal Boeckx ; Kathy SteppeSource :
- Physiologia plantarum [ 1399-3054 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Isotopes du carbone, Radio-isotopes du carbone.
- métabolisme : Dioxyde de carbone, Feuilles de plante, Populus, Xylème.
- Arbres, Transport biologique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Carbon Isotopes, Carbon Radioisotopes.
- chemical , metabolism : Carbon Dioxide.
- metabolism : Plant Leaves, Populus, Xylem.
- Biological Transport, Trees.
Abstract
In recent studies, assimilation of xylem-transported CO2 has gained considerable attention as a means of recycling respired CO2 in trees. However, we still lack a clear and detailed picture on the magnitude of xylem-transported CO2 assimilation, in particular within leaf tissues. To this end, detached poplar leaves (Populus × canadensis Moench 'Robusta') were allowed to take up a dissolved (13)CO2 label serving as a proxy of xylem-transported CO2 entering the leaf from the branch. The uptake rate of the (13)C was manipulated by altering the vapor pressure deficit (VPD) (0.84, 1.29 and 1.83 kPa). Highest tissue enrichments were observed under the highest VPD. Among tissues, highest enrichment was observed in the petiole and the veins, regardless of the VPD treatment. Analysis of non-labeled leaves showed that some (13)C diffused from the labeled leaves and was fixed in the mesophyll of the non-labeled leaves. However, (13)C leaf tissue enrichment analysis with elemental analysis coupled to isotope ratio mass spectrometry was limited in spatial resolution at the leaf tissue level. Therefore, (11)C-based CO2 labeling combined with positron autoradiography was used and showed a more detailed spatial distribution within a single tissue, in particular in secondary veins. Therefore, in addition to (13)C, (11) C-based autoradiography can be used to study the fate of xylem-transported CO2 at leaf level, allowing the acquisition of data at a yet unprecedented resolution.
DOI: 10.1111/ppl.12262
PubMed: 25142926
Affiliations:
Links toward previous steps (curation, corpus...)
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Vos, Filip" uniqKey="De Vos F" first="Filip" last="De Vos">Filip De Vos</name></author><author><name sortKey="Vanhove, Christian" sort="Vanhove, Christian" uniqKey="Vanhove C" first="Christian" last="Vanhove">Christian Vanhove</name></author><author><name sortKey="Vandenberghe, Stefaan" sort="Vandenberghe, Stefaan" uniqKey="Vandenberghe S" first="Stefaan" last="Vandenberghe">Stefaan Vandenberghe</name></author><author><name sortKey="Boeckx, Pascal" sort="Boeckx, Pascal" uniqKey="Boeckx P" first="Pascal" last="Boeckx">Pascal Boeckx</name></author><author><name sortKey="Steppe, Kathy" sort="Steppe, Kathy" uniqKey="Steppe K" first="Kathy" last="Steppe">Kathy Steppe</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25142926</idno><idno type="pmid">25142926</idno><idno type="doi">10.1111/ppl.12262</idno><idno type="wicri:Area/Main/Corpus">002042</idno><idno type="wicri:explorRef" wicri:stream="Main" 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Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Bauweraerts, Ingvar" sort="Bauweraerts, Ingvar" uniqKey="Bauweraerts I" first="Ingvar" last="Bauweraerts">Ingvar Bauweraerts</name></author><author><name sortKey="De Vos, Filip" sort="De Vos, Filip" uniqKey="De Vos F" first="Filip" last="De Vos">Filip De Vos</name></author><author><name sortKey="Vanhove, Christian" sort="Vanhove, Christian" uniqKey="Vanhove C" first="Christian" last="Vanhove">Christian Vanhove</name></author><author><name sortKey="Vandenberghe, Stefaan" sort="Vandenberghe, Stefaan" uniqKey="Vandenberghe S" first="Stefaan" last="Vandenberghe">Stefaan Vandenberghe</name></author><author><name sortKey="Boeckx, Pascal" sort="Boeckx, Pascal" uniqKey="Boeckx P" first="Pascal" last="Boeckx">Pascal Boeckx</name></author><author><name sortKey="Steppe, Kathy" sort="Steppe, Kathy" uniqKey="Steppe K" first="Kathy" last="Steppe">Kathy Steppe</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Transport (MeSH)</term><term>Carbon Dioxide (metabolism)</term><term>Carbon Isotopes (analysis)</term><term>Carbon Radioisotopes (analysis)</term><term>Plant Leaves (metabolism)</term><term>Populus (metabolism)</term><term>Trees (MeSH)</term><term>Xylem (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (MeSH)</term><term>Dioxyde de carbone (métabolisme)</term><term>Feuilles de plante (métabolisme)</term><term>Isotopes du carbone (analyse)</term><term>Populus (métabolisme)</term><term>Radio-isotopes du carbone (analyse)</term><term>Transport biologique (MeSH)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbon Isotopes</term><term>Carbon Radioisotopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Isotopes du carbone</term><term>Radio-isotopes du carbone</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dioxyde de carbone</term><term>Feuilles de plante</term><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In recent studies, assimilation of xylem-transported CO2 has gained considerable attention as a means of recycling respired CO2 in trees. However, we still lack a clear and detailed picture on the magnitude of xylem-transported CO2 assimilation, in particular within leaf tissues. To this end, detached poplar leaves (Populus × canadensis Moench 'Robusta') were allowed to take up a dissolved (13)CO2 label serving as a proxy of xylem-transported CO2 entering the leaf from the branch. The uptake rate of the (13)C was manipulated by altering the vapor pressure deficit (VPD) (0.84, 1.29 and 1.83 kPa). Highest tissue enrichments were observed under the highest VPD. Among tissues, highest enrichment was observed in the petiole and the veins, regardless of the VPD treatment. Analysis of non-labeled leaves showed that some (13)C diffused from the labeled leaves and was fixed in the mesophyll of the non-labeled leaves. However, (13)C leaf tissue enrichment analysis with elemental analysis coupled to isotope ratio mass spectrometry was limited in spatial resolution at the leaf tissue level. Therefore, (11)C-based CO2 labeling combined with positron autoradiography was used and showed a more detailed spatial distribution within a single tissue, in particular in secondary veins. Therefore, in addition to (13)C, (11) C-based autoradiography can be used to study the fate of xylem-transported CO2 at leaf level, allowing the acquisition of data at a yet unprecedented resolution.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25142926</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>03</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>153</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Fate of xylem-transported 11C- and 13C-labeled CO2 in leaves of poplar.</ArticleTitle><Pagination><MedlinePgn>555-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12262</ELocationID><Abstract><AbstractText>In recent studies, assimilation of xylem-transported CO2 has gained considerable attention as a means of recycling respired CO2 in trees. However, we still lack a clear and detailed picture on the magnitude of xylem-transported CO2 assimilation, in particular within leaf tissues. To this end, detached poplar leaves (Populus × canadensis Moench 'Robusta') were allowed to take up a dissolved (13)CO2 label serving as a proxy of xylem-transported CO2 entering the leaf from the branch. The uptake rate of the (13)C was manipulated by altering the vapor pressure deficit (VPD) (0.84, 1.29 and 1.83 kPa). Highest tissue enrichments were observed under the highest VPD. Among tissues, highest enrichment was observed in the petiole and the veins, regardless of the VPD treatment. Analysis of non-labeled leaves showed that some (13)C diffused from the labeled leaves and was fixed in the mesophyll of the non-labeled leaves. However, (13)C leaf tissue enrichment analysis with elemental analysis coupled to isotope ratio mass spectrometry was limited in spatial resolution at the leaf tissue level. Therefore, (11)C-based CO2 labeling combined with positron autoradiography was used and showed a more detailed spatial distribution within a single tissue, in particular in secondary veins. Therefore, in addition to (13)C, (11) C-based autoradiography can be used to study the fate of xylem-transported CO2 at leaf level, allowing the acquisition of data at a yet unprecedented resolution.</AbstractText><CopyrightInformation>© 2014 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bloemen</LastName><ForeName>Jasper</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Ecology, Ghent University, 9000, Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bauweraerts</LastName><ForeName>Ingvar</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>De Vos</LastName><ForeName>Filip</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Vanhove</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Vandenberghe</LastName><ForeName>Stefaan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Boeckx</LastName><ForeName>Pascal</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Steppe</LastName><ForeName>Kathy</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>10</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002250">Carbon Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002250" MajorTopicYN="N">Carbon Radioisotopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>07</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25142926</ArticleId><ArticleId IdType="doi">10.1111/ppl.12262</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Bauweraerts, Ingvar" sort="Bauweraerts, Ingvar" uniqKey="Bauweraerts I" first="Ingvar" last="Bauweraerts">Ingvar Bauweraerts</name><name sortKey="Boeckx, Pascal" sort="Boeckx, Pascal" uniqKey="Boeckx P" first="Pascal" last="Boeckx">Pascal Boeckx</name><name sortKey="De Vos, Filip" sort="De Vos, Filip" uniqKey="De Vos F" first="Filip" last="De Vos">Filip De Vos</name><name sortKey="Steppe, Kathy" sort="Steppe, Kathy" uniqKey="Steppe K" first="Kathy" last="Steppe">Kathy Steppe</name><name sortKey="Vandenberghe, Stefaan" sort="Vandenberghe, Stefaan" uniqKey="Vandenberghe S" first="Stefaan" last="Vandenberghe">Stefaan Vandenberghe</name><name sortKey="Vanhove, Christian" sort="Vanhove, Christian" uniqKey="Vanhove C" first="Christian" last="Vanhove">Christian Vanhove</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Bloemen, Jasper" sort="Bloemen, Jasper" uniqKey="Bloemen J" first="Jasper" last="Bloemen">Jasper Bloemen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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